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B3GNT2 ELISA Kit
B3GNT2
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881915
Produktdetails B3GNT2 ELISA Kit
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Target
Alle B3GNT2 Produkte
B3GNT2
(UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human B3GNT2 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu B3GNT2
(ausblenden)
Target
Alle B3GNT2 Produkte
B3GNT2
(UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
Andere Bezeichnung
B3GNT2 (B3GNT2 Produkte )
Synonyme
B3GN-T2 ELISA Kit, B3GNT ELISA Kit, B3GNT-2 ELISA Kit, B3GNT1 ELISA Kit, BETA3GNT ELISA Kit, BGNT2 ELISA Kit, BGnT-2 ELISA Kit, AA408337 ELISA Kit, AA409404 ELISA Kit, AW260308 ELISA Kit, B3Galt6 ELISA Kit, B3gnt1 ELISA Kit, BGnT-1 ELISA Kit, BGnT1 ELISA Kit, BGnT2 ELISA Kit, Beta3gnt ELISA Kit, MGC81185 ELISA Kit, B3GNT2 ELISA Kit, MGC179257 ELISA Kit, ssp5 ELISA Kit, wu:fa55h04 ELISA Kit, wu:fc39c01 ELISA Kit, zgc:113947 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 L homeolog ELISA Kit, beta-1,4-glucuronyltransferase 1 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2b ELISA Kit, B3GNT2 ELISA Kit, B3gnt2 ELISA Kit, b3gnt2.L ELISA Kit, B4GAT1 ELISA Kit, b3gnt2b ELISA Kit, b3gnt2 ELISA Kit
Gen-ID
10678
UniProt
Q9NY97
Pathways
Glycosaminoglycan Metabolic Process
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