CANT1 ELISA Kit

Produktnummer ABIN4882079

Produktdetails CANT1 ELISA Kit

Target: CANT1 (Calcium Activated Nucleotidase 1 (CANT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human CANT1/SCAN-1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human FABP4.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CANT1

Target: CANT1 (Calcium Activated Nucleotidase 1 (CANT1))

Andere Bezeichnung: CANT1 (CANT1 Produkte)

Synonyme: DBQD ELISA Kit, SCAN-1 ELISA Kit, SCAN1 ELISA Kit, SHAPY ELISA Kit, 5830420C20Rik ELISA Kit, Apy1h ELISA Kit, D11Bwg0554e ELISA Kit, Entpd8 ELISA Kit, Shapy ELISA Kit, srapy ELISA Kit, apyrase ELISA Kit, xapy ELISA Kit, cant1 ELISA Kit, entpd8 ELISA Kit, zgc:85724 ELISA Kit, CANT1 ELISA Kit, zgc:101021 ELISA Kit, calcium activated nucleotidase 1 ELISA Kit, calcium activated nucleotidase 1 L homeolog ELISA Kit, calcium activated nucleotidase 1a ELISA Kit, calcium activated nucleotidase 1b ELISA Kit, CANT1 ELISA Kit, Cant1 ELISA Kit, cant1.L ELISA Kit, cant1 ELISA Kit, cant1a ELISA Kit, LOAG_01305 ELISA Kit, cant1b ELISA Kit

Gen-ID: 124583

UniProt: Q8WVQ1