CPB1 ELISA Kit

Produktnummer ABIN4882085

Produktdetails CPB1 ELISA Kit

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Carboxypeptidase B1 (CPB1) ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Andere Bezeichnung: Carboxypeptidase B1 (CPB1 Produkte)

Synonyme: wu:fb60d07 ELISA Kit, wu:fb69b08 ELISA Kit, CPB1 ELISA Kit, pCPB ELISA Kit, CPB ELISA Kit, PASP ELISA Kit, PCPB ELISA Kit, Cpb ELISA Kit, 0910001A18Rik ELISA Kit, 1810063F02Rik ELISA Kit, 2210008M23Rik ELISA Kit, AI504870 ELISA Kit, carboxypeptidase B1 (tissue) ELISA Kit, carboxypeptidase B1 ELISA Kit, carboxypeptidase B1 S homeolog ELISA Kit, carboxypeptidase B ELISA Kit, cpb1 ELISA Kit, CPB1 ELISA Kit, cpb1.S ELISA Kit, CpipJ_CPIJ010799 ELISA Kit, CpipJ_CPIJ010801 ELISA Kit, VDBG_02663 ELISA Kit, LOC100562609 ELISA Kit, Cpb1 ELISA Kit

Gen-ID: 1360

UniProt: P15086