BIRC2 ELISA Kit

Produktnummer ABIN4882354

Produktdetails BIRC2 ELISA Kit

Target: BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human cIAP-1 (BIRC2/hIAP-2) ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu BIRC2

Target: BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))

Andere Bezeichnung: cIAP-1 (BIRC2 Produkte)

Synonyme: API1 ELISA Kit, HIAP2 ELISA Kit, Hiap-2 ELISA Kit, MIHB ELISA Kit, RNF48 ELISA Kit, c-IAP1 ELISA Kit, cIAP1 ELISA Kit, Api2 ELISA Kit, rIAP1 ELISA Kit, IAP1 ELISA Kit, ITA ELISA Kit, AW146227 ELISA Kit, Api1 ELISA Kit, Birc3 ELISA Kit, HIAP1 ELISA Kit, IAP2 ELISA Kit, MIAP1 ELISA Kit, MIAP2 ELISA Kit, MIHC ELISA Kit, cIAP2 ELISA Kit, mcIAP1 ELISA Kit, ciap-1 ELISA Kit, birc3 ELISA Kit, api1 ELISA Kit, birc2 ELISA Kit, c-iap1 ELISA Kit, ciap1 ELISA Kit, hiap-2 ELISA Kit, hiap2 ELISA Kit, mihb ELISA Kit, rnf48 ELISA Kit, baculoviral IAP repeat containing 2 ELISA Kit, baculoviral IAP repeat-containing 2 ELISA Kit, baculoviral IAP repeat containing 2 L homeolog ELISA Kit, BIRC2 ELISA Kit, Birc2 ELISA Kit, birc2 ELISA Kit, birc2.L ELISA Kit

Gen-ID: 329

UniProt: Q13490

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades