c-MYC ELISA Kit

Produktnummer ABIN4882399

Produktdetails c-MYC ELISA Kit

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.62-150 ng/mL

Untere Nachweisgrenze: 0.62 ng/mL

Applikation: ELISA

Verwendungszweck: Human c-Myc (MYC) ELISA Kit for serum, plasma, and cell culture supernatant samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human c-Myc. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Andere Bezeichnung: C-Myc///MYC (MYC Produkte)

Synonyme: MRTL ELISA Kit, MYCC ELISA Kit, bHLHe39 ELISA Kit, c-Myc ELISA Kit, CMYC ELISA Kit, c-myc ELISA Kit, C-MYC ELISA Kit, LOC100136746 ELISA Kit, ARC ELISA Kit, B430311C09Rik ELISA Kit, MYC ELISA Kit, NOP ELISA Kit, Nop30 ELISA Kit, RNCMYC ELISA Kit, mMyc ELISA Kit, AU016757 ELISA Kit, Myc2 ELISA Kit, Niard ELISA Kit, Nird ELISA Kit, cmyc ELISA Kit, zc-myc ELISA Kit, c-myc II ELISA Kit, myc-B ELISA Kit, myc2 ELISA Kit, myc1 ELISA Kit, CMyc ELISA Kit, zgc:55680 ELISA Kit, CG10798 ELISA Kit, D-Myc ELISA Kit, DM ELISA Kit, DMYc ELISA Kit, Dm ELISA Kit, Dmel\CG10798 ELISA Kit, Dmyc ELISA Kit, EG:BACN5I9.1 ELISA Kit, anon-WO03040301.171 ELISA Kit, bHLHe57 ELISA Kit, c-MYC ELISA Kit, d-myc ELISA Kit, dMYC ELISA Kit, dMyc ELISA Kit, dMyc1 ELISA Kit, da ELISA Kit, dm ELISA Kit, dm/dMyc ELISA Kit, dm/myc ELISA Kit, dmyc ELISA Kit, dmyc1 ELISA Kit, l(1)G0139 ELISA Kit, l(1)G0354 ELISA Kit, l(1)G0359 ELISA Kit, myc ELISA Kit, MYC proto-oncogene, bHLH transcription factor ELISA Kit, v-myc myelocytomatosis viral oncogene homolog ELISA Kit, nucleolar protein 3 (apoptosis repressor with CARD domain) ELISA Kit, myelocytomatosis oncogene ELISA Kit, MYC proto-oncogene, bHLH transcription factor a ELISA Kit, MYC proto-oncogene, bHLH transcription factor L homeolog ELISA Kit, MYC proto-oncogene, bHLH transcription factor S homeolog ELISA Kit, v-myc myelocytomatosis viral oncogene homolog (avian) ELISA Kit, MYC proto-oncogene, bHLH transcription factor b ELISA Kit, CG10798 gene product from transcript CG10798-RB ELISA Kit, MYC ELISA Kit, LOC100136746 ELISA Kit, Nol3 ELISA Kit, Myc ELISA Kit, myca ELISA Kit, myc.L ELISA Kit, myc.S ELISA Kit, mycb ELISA Kit

Gen-ID: 4609

UniProt: P01106

Pathways: p53 Signalweg, Zellzyklus, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effekt