CSTB ELISA Kit

Produktnummer ABIN4882533

Produktdetails CSTB ELISA Kit

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Cystatin B (CSTB) ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human Cystatin B (CSTB)

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Andere Bezeichnung: Cystatin B (CSTB Produkte)

Synonyme: CST6 ELISA Kit, EPM1 ELISA Kit, EPM1A ELISA Kit, PME ELISA Kit, STFB ELISA Kit, ULD ELISA Kit, CSTB ELISA Kit, Cyb ELISA Kit, Epm1 ELISA Kit, Stfb ELISA Kit, MGC189005 ELISA Kit, pme ELISA Kit, cst6 ELISA Kit, epm1 ELISA Kit, stfb ELISA Kit, AA960480 ELISA Kit, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 ELISA Kit, ATCYS6 ELISA Kit, ATCYSB ELISA Kit, cystatin B ELISA Kit, LOC100008600 ELISA Kit, LOC100136235 ELISA Kit, CPI1 ELISA Kit, cystatin B ELISA Kit, cystatin B (stefin B) ELISA Kit, cystatin 14a, tandem duplicate 2 ELISA Kit, cystatin-B ELISA Kit, CSTB ELISA Kit, Cstb ELISA Kit, cstb ELISA Kit, cst14a.2 ELISA Kit, CYSB ELISA Kit, cpi1 ELISA Kit, LOC101123265 ELISA Kit

Gen-ID: 1476

UniProt: P04080

Pathways: Response to Water Deprivation