DDR1 ELISA Kit

Produktnummer ABIN4882584

Produktdetails DDR1 ELISA Kit

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human DDR1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu DDR1

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Andere Bezeichnung: DDR1 (DDR1 Produkte)

Synonyme: CG11573 ELISA Kit, CG13984 ELISA Kit, CG13985 ELISA Kit, CG33531 ELISA Kit, CG9488 ELISA Kit, CG9490 ELISA Kit, CT33540 ELISA Kit, DDR ELISA Kit, Dmel\\CG33531 ELISA Kit, Dmel_CG13984 ELISA Kit, 6030432F18 ELISA Kit, AI323681 ELISA Kit, CD167a ELISA Kit, Cak ELISA Kit, Nep ELISA Kit, PTK3A ELISA Kit, CAK ELISA Kit, CD167 ELISA Kit, EDDR1 ELISA Kit, HGK2 ELISA Kit, MCK10 ELISA Kit, NEP ELISA Kit, NTRK4 ELISA Kit, PTK3 ELISA Kit, RTK6 ELISA Kit, TRKE ELISA Kit, Drd1 ELISA Kit, PTK3D ELISA Kit, Discoidin domain receptor ELISA Kit, discoidin domain receptor family, member 1 ELISA Kit, discoidin domain receptor tyrosine kinase 1 ELISA Kit, Ddr ELISA Kit, Ddr1 ELISA Kit, DDR1 ELISA Kit

Gen-ID: 780

UniProt: Q08345

Pathways: RTK Signalweg, Smooth Muscle Cell Migration