Dynamitin ELISA Kit

Produktnummer ABIN4882620

Produktdetails Dynamitin ELISA Kit

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Dynactin subunit 2/DCTN2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Protocadherin-17.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Dynamitin

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Andere Bezeichnung: Dynactin Subunit 2 (DCTN2 Produkte)

Synonyme: DCTN2 ELISA Kit, dctn2 ELISA Kit, dctn50 ELISA Kit, dynamitin ELISA Kit, rbp50 ELISA Kit, CG8269 ELISA Kit, DMN ELISA Kit, Dmel\\CG8269 ELISA Kit, P50 ELISA Kit, dDyn ELISA Kit, dmn ELISA Kit, dyna ELISA Kit, l(2)k16109 ELISA Kit, l(2)k16218 ELISA Kit, p50 ELISA Kit, p50/Dmn ELISA Kit, p50/dynamitin ELISA Kit, 2310042E05Rik ELISA Kit, C130077D06Rik ELISA Kit, C87049 ELISA Kit, DCTN-50 ELISA Kit, GMP23-48K ELISA Kit, RBP50 ELISA Kit, DCTN50 ELISA Kit, DYNAMITIN ELISA Kit, zgc:63867 ELISA Kit, dynactin subunit 2 ELISA Kit, dynactin subunit 2 S homeolog ELISA Kit, Dynactin 2, p50 subunit ELISA Kit, dynactin 2 ELISA Kit, dynactin 2 (p50) ELISA Kit, dynactin subunit 2 L homeolog ELISA Kit, DCTN2 ELISA Kit, dctn2.S ELISA Kit, DCTN2-p50 ELISA Kit, dctn2 ELISA Kit, Dctn2 ELISA Kit, dctn2.L ELISA Kit

Gen-ID: 10540

UniProt: Q13561

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization