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EGR1 ELISA Kit
EGR1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4882701
Produktdetails EGR1 ELISA Kit
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Target
Alle EGR1 ELISA Kits anzeigen
EGR1
(Early Growth Response 1 (EGR1))
Reaktivität
Alle Reaktivitäten für EGR1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Human EGR1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human EGR1.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu EGR1
(ausblenden)
Target
Alle EGR1 ELISA Kits anzeigen
EGR1
(Early Growth Response 1 (EGR1))
Andere Bezeichnung
EGR1 (EGR1 Produkte )
Synonyme
AT225 ELISA Kit, G0S30 ELISA Kit, KROX-24 ELISA Kit, NGFI-A ELISA Kit, TIS8 ELISA Kit, ZIF-268 ELISA Kit, ZNF225 ELISA Kit, A530045N19Rik ELISA Kit, ETR103 ELISA Kit, Egr-1 ELISA Kit, Krox-1 ELISA Kit, Krox-24 ELISA Kit, Krox24 ELISA Kit, NGF1-A ELISA Kit, NGFIA ELISA Kit, Zenk ELISA Kit, Zfp-6 ELISA Kit, Zif268 ELISA Kit, egr ELISA Kit, Ngf1 ELISA Kit, Ngfi ELISA Kit, zif-268 ELISA Kit, EGR1 ELISA Kit, EGR-1-A ELISA Kit, Xegr-1 ELISA Kit, at225 ELISA Kit, egr-1 ELISA Kit, egr1 ELISA Kit, g0s30 ELISA Kit, krox-24 ELISA Kit, ngfi-a ELISA Kit, tis8 ELISA Kit, znf225 ELISA Kit, krox24 ELISA Kit, wu:fj64b05 ELISA Kit, wu:fq25f01 ELISA Kit, EGR-1 ELISA Kit, zenk ELISA Kit, EGR-1-B ELISA Kit, egr1-a ELISA Kit, egr1-b ELISA Kit, early growth response 1 ELISA Kit, early growth response protein 1 (egr-1) ELISA Kit, early growth response 1 L homeolog ELISA Kit, early growth response 1 S homeolog ELISA Kit, EGR1 ELISA Kit, CNK00890 ELISA Kit, Egr1 ELISA Kit, egr1.L ELISA Kit, egr1 ELISA Kit, egr1.S ELISA Kit
Gen-ID
1958
UniProt
P18146
Pathways
Regulation of Carbohydrate Metabolic Process , Regulation of long-term Neuronal Synaptic Plasticity
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