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TMPRSS15 ELISA Kit
TMPRSS15
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4882731
Produktdetails TMPRSS15 ELISA Kit
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Target
Alle TMPRSS15 ELISA Kits anzeigen
TMPRSS15
(Transmembrane Protease, serine 15 (TMPRSS15))
Reaktivität
Alle Reaktivitäten für TMPRSS15 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Enteropeptidase ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TMPRSS15
(ausblenden)
Target
Alle TMPRSS15 ELISA Kits anzeigen
TMPRSS15
(Transmembrane Protease, serine 15 (TMPRSS15))
Andere Bezeichnung
Enteropeptidase (TMPRSS15 Produkte )
Synonyme
CG3066 ELISA Kit, Dmel\\CG3066 ELISA Kit, MP2 ELISA Kit, PAE1 ELISA Kit, SP7 ELISA Kit, anon-Ryu ELISA Kit, proPO-AE ELISA Kit, sp7 ELISA Kit, NV14771 ELISA Kit, PRSS7 ELISA Kit, ENTK ELISA Kit, Entk ELISA Kit, Prss7 ELISA Kit, Serine protease 7 ELISA Kit, serine protease 7 ELISA Kit, transmembrane protease, serine 7 ELISA Kit, transmembrane protease, serine 15 ELISA Kit, Sp7 ELISA Kit, LOC733125 ELISA Kit, SP7 ELISA Kit, TMPRSS7 ELISA Kit, TMPRSS15 ELISA Kit, Tmprss15 ELISA Kit
Gen-ID
5651
UniProt
P98073
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