EXTL3 ELISA Kit

Produktnummer ABIN4882781

Produktdetails EXTL3 ELISA Kit

Target: EXTL3 (Exostoses (Multiple)-Like 3 (EXTL3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Exostosin-like 3/EXTL3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human TRP14.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu EXTL3

Target: EXTL3 (Exostoses (Multiple)-Like 3 (EXTL3))

Andere Bezeichnung: Exostosin-Like 3 (EXTL3 Produkte)

Synonyme: BcDNA:LD21192 ELISA Kit, Botv ELISA Kit, CG15110 ELISA Kit, DEXT3 ELISA Kit, DEXTL3 ELISA Kit, Dextl3 ELISA Kit, Dmel\\CG15110 ELISA Kit, Extl3 ELISA Kit, 2900009G18Rik ELISA Kit, AU040105 ELISA Kit, Ext1l ELISA Kit, mKIAA0519 ELISA Kit, BOTV ELISA Kit, EXTL1L ELISA Kit, EXTR1 ELISA Kit, REGR ELISA Kit, RPR ELISA Kit, Regr ELISA Kit, brother of tout-velu ELISA Kit, exostoses (multiple)-like 3 ELISA Kit, exostosin like glycosyltransferase 3 ELISA Kit, exostosin-like glycosyltransferase 3 ELISA Kit, botv ELISA Kit, Extl3 ELISA Kit, EXTL3 ELISA Kit

Gen-ID: 2137

UniProt: O43909

Pathways: Glycosaminoglycan Metabolic Process, ER-Nucleus Signaling