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FKBP3 ELISA Kit

FKBP3 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4882888
  • Target Alle FKBP3 ELISA Kits anzeigen
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human FKBP25/FKBP3 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes .
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle FKBP3 ELISA Kits anzeigen
    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))
    Andere Bezeichnung
    FKBP25 (FKBP3 Produkte)
    Synonyme
    FKBP3 ELISA Kit, 25kDa ELISA Kit, FKBP-3 ELISA Kit, FKBP25 ELISA Kit, FK506 ELISA Kit, fb75c04 ELISA Kit, si:dz261o22.2 ELISA Kit, wu:fb75c04 ELISA Kit, zgc:110728 ELISA Kit, FKBP-25 ELISA Kit, PPIase ELISA Kit, FK506 binding protein 3 ELISA Kit, FK506 binding protein 3 L homeolog ELISA Kit, peptidylprolyl isomerase ELISA Kit, FKBP3 ELISA Kit, Fkbp3 ELISA Kit, fkbp3 ELISA Kit, fkbp3.L ELISA Kit, CAALFM_C103790CA ELISA Kit
    Gen-ID
    2287
    UniProt
    Q00688
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