FUCA1 ELISA Kit

Produktnummer ABIN4882951

Produktdetails FUCA1 ELISA Kit

Target: FUCA1 (Fucosidase, alpha-L- 1, Tissue (FUCA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human FUCA1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu FUCA1

Target: FUCA1 (Fucosidase, alpha-L- 1, Tissue (FUCA1))

Andere Bezeichnung: FUCA1 (FUCA1 Produkte)

Synonyme: FUCA ELISA Kit, FUCA1 ELISA Kit, 0610006A03Rik ELISA Kit, 9530055J05Rik ELISA Kit, Afuc ELISA Kit, Fuca ELISA Kit, fuca ELISA Kit, fuca1 ELISA Kit, ATFUC1 ELISA Kit, F24D13.11 ELISA Kit, F24D13_11 ELISA Kit, alpha-L-fucosidase 1 ELISA Kit, wu:fb02a09 ELISA Kit, zgc:101116 ELISA Kit, alpha-L-fucosidase 1 ELISA Kit, fucosidase, alpha-L- 1, tissue ELISA Kit, alpha-L-fucosidase 1 L homeolog ELISA Kit, alpha-L-fucosidase 1, tandem duplicate 2 ELISA Kit, FUCA1 ELISA Kit, Fuca1 ELISA Kit, fuca1.L ELISA Kit, fuca1 ELISA Kit, FUC1 ELISA Kit, LOC9317020 ELISA Kit, LOC100285202 ELISA Kit, fuca1.2 ELISA Kit

Gen-ID: 2517

UniProt: P04066

Pathways: Glycosaminoglycan Metabolic Process