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WFIKKN2 ELISA Kit
WFIKKN2
Reaktivität: Human
Colorimetric
Sandwich ELISA
20-5000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883008
Produktdetails WFIKKN2 ELISA Kit
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Target
Alle WFIKKN2 ELISA Kits anzeigen
WFIKKN2
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2))
Reaktivität
Alle Reaktivitäten für WFIKKN2 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
20-5000 pg/mL
Untere Nachweisgrenze
20 pg/mL
Applikation
ELISA
Verwendungszweck
Human GASP-1 (WFIKKNRP) ELISA Kit for serum, plasma, and cell culture supernatant samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE-2, aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples10 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu WFIKKN2
(ausblenden)
Target
Alle WFIKKN2 ELISA Kits anzeigen
WFIKKN2
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2))
Andere Bezeichnung
GASP-1///WFIKKNRP (WFIKKN2 Produkte )
Synonyme
GASP-1 ELISA Kit, WFDC20B ELISA Kit, WFIKKNRP ELISA Kit, hGASP-1 ELISA Kit, 2610304F08Rik ELISA Kit, AY100450 ELISA Kit, Gasp1 ELISA Kit, WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 ELISA Kit, WFIKKN2 ELISA Kit, Wfikkn2 ELISA Kit
Gen-ID
9737
UniProt
Q5JY77
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