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WFIKKN1 ELISA Kit
WFIKKN1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883011
Produktdetails WFIKKN1 ELISA Kit
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Target
Alle WFIKKN1 ELISA Kits anzeigen
WFIKKN1
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 1 (WFIKKN1))
Reaktivität
Alle Reaktivitäten für WFIKKN1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human GASP-2 (WFIKKN) ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes human GASP-2/WFIKKN.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu WFIKKN1
(ausblenden)
Target
Alle WFIKKN1 ELISA Kits anzeigen
WFIKKN1
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 1 (WFIKKN1))
Andere Bezeichnung
GASP-2 (WFIKKN1 Produkte )
Synonyme
C16orf12 ELISA Kit, RJD2 ELISA Kit, WFDC20A ELISA Kit, WFIKKN ELISA Kit, Oc29 ELISA Kit, RGD1565835 ELISA Kit, WFIKKN1 ELISA Kit, Wfikkn ELISA Kit, Gasp2 ELISA Kit, wfikkn ELISA Kit, zgc:85816 ELISA Kit, WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 ELISA Kit, WAP, FS, Ig, KU, and NTR-containing protein 1 ELISA Kit, WFIKKN1 ELISA Kit, Wfikkn1 ELISA Kit, wfikkn1 ELISA Kit
Gen-ID
100528062, 114928
UniProt
Q96D09
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