GLI3 ELISA Kit

Produktnummer ABIN4883079

Produktdetails GLI3 ELISA Kit

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human GLI-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human GLI-3.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Andere Bezeichnung: GLI-3 (GLI3 Produkte)

Synonyme: ACLS ELISA Kit, GCPS ELISA Kit, GLI3-190 ELISA Kit, GLI3FL ELISA Kit, PAP-A ELISA Kit, PAPA ELISA Kit, PAPA1 ELISA Kit, PAPB ELISA Kit, PHS ELISA Kit, PPDIV ELISA Kit, xgli3 ELISA Kit, AI854843 ELISA Kit, AU023367 ELISA Kit, Bph ELISA Kit, Pdn ELISA Kit, Xt ELISA Kit, add ELISA Kit, im:7142603 ELISA Kit, GLI3 ELISA Kit, GLI family zinc finger 3 ELISA Kit, GLI family zinc finger 3 L homeolog ELISA Kit, GLI-Kruppel family member GLI3 ELISA Kit, GLI3 ELISA Kit, Gli3 ELISA Kit, gli3.L ELISA Kit, gli3 ELISA Kit

Gen-ID: 2737

UniProt: P10071

Pathways: Hedgehog Signalweg