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HAGH ELISA Kit

HAGH Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883097
  • Target Alle HAGH ELISA Kits anzeigen
    HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
    Reaktivität
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Glyoxalase II ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes .
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle HAGH ELISA Kits anzeigen
    HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
    Andere Bezeichnung
    Glyoxalase II (HAGH Produkte)
    Synonyme
    glo-2 ELISA Kit, glo2 ELISA Kit, glx2 ELISA Kit, glxii ELISA Kit, hagh1 ELISA Kit, DDBDRAFT_0186675 ELISA Kit, DDBDRAFT_0230988 ELISA Kit, DDB_0186675 ELISA Kit, DDB_0230988 ELISA Kit, DDBDRAFT_0183924 ELISA Kit, DDBDRAFT_0230991 ELISA Kit, DDB_0183924 ELISA Kit, DDB_0230991 ELISA Kit, BC019817 ELISA Kit, Glo-2 ELISA Kit, Glo2 ELISA Kit, Rsp29 ELISA Kit, RSP29 ELISA Kit, fa66a03 ELISA Kit, wu:fa66a03 ELISA Kit, zgc:73161 ELISA Kit, GLO2 ELISA Kit, GLX2 ELISA Kit, GLXII ELISA Kit, HAGH1 ELISA Kit, hydroxyacylglutathione hydrolase L homeolog ELISA Kit, hydroxyacylglutathione hydrolase ELISA Kit, beta-lactamase domain-containing protein ELISA Kit, hydroxyacyl glutathione hydrolase ELISA Kit, hagh.L ELISA Kit, CND02510 ELISA Kit, gloB1 ELISA Kit, gloB2 ELISA Kit, Fbal_1466 ELISA Kit, Hagh ELISA Kit, hagh ELISA Kit, HAGH ELISA Kit
    Gen-ID
    3029
    UniProt
    Q16775
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