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IL28RA ELISA Kit
IL28RA
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883402
Produktdetails IL28RA ELISA Kit
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Target
Alle IL28RA ELISA Kits anzeigen
IL28RA
(Interleukin 28 Receptor, alpha (Interferon, lambda Receptor) (IL28RA))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human IL-28 R alpha (IFN-lambda R1) ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu IL28RA
(ausblenden)
Target
Alle IL28RA ELISA Kits anzeigen
IL28RA
(Interleukin 28 Receptor, alpha (Interferon, lambda Receptor) (IL28RA))
Andere Bezeichnung
IL-28 R alpha (IL28RA Produkte )
Synonyme
IFNLR1 ELISA Kit, IL28RA ELISA Kit, crf2/12 ELISA Kit, ifnlr ELISA Kit, il-28r1 ELISA Kit, il28ra ELISA Kit, licr2 ELISA Kit, ifnlr1 ELISA Kit, CRF2-12 ELISA Kit, Il28ra ELISA Kit, CRF2/12 ELISA Kit, IFNLR ELISA Kit, IL-28R1 ELISA Kit, LICR2 ELISA Kit, RGD1562689 ELISA Kit, interferon lambda receptor 1 ELISA Kit, interferon, lambda receptor 1 ELISA Kit, interferon, lambda receptor 1 L homeolog ELISA Kit, IFNLR1 ELISA Kit, ifnlr1 ELISA Kit, ifnlr1.L ELISA Kit, Ifnlr1 ELISA Kit
Gen-ID
163702
UniProt
Q8IU57
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