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ING1 ELISA Kit
ING1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883452
Produktdetails ING1 ELISA Kit
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Target
Alle ING1 ELISA Kits anzeigen
ING1
(Inhibitor of Growth Family, Member 1 (ING1))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human ING1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu ING1
(ausblenden)
Target
Alle ING1 ELISA Kits anzeigen
ING1
(Inhibitor of Growth Family, Member 1 (ING1))
Andere Bezeichnung
ING1 (ING1 Produkte )
Synonyme
p24ING1c ELISA Kit, p33 ELISA Kit, p33ING1 ELISA Kit, p33ING1b ELISA Kit, p47 ELISA Kit, p47ING1a ELISA Kit, p33ING1c ELISA Kit, zgc:136918 ELISA Kit, ING1 ELISA Kit, 2610028J21Rik ELISA Kit, AA407184 ELISA Kit, AI875420 ELISA Kit, mING1h ELISA Kit, p33Ing1 ELISA Kit, p37Ing1b ELISA Kit, xing1 ELISA Kit, inhibitor of growth family member 1 ELISA Kit, inhibitor of growth family, member 1 ELISA Kit, inhibitor of growth family member 1 S homeolog ELISA Kit, ING1 ELISA Kit, Ing1 ELISA Kit, ing1 ELISA Kit, ing1.S ELISA Kit
Gen-ID
3621
UniProt
Q9UK53
Pathways
Protein targeting to Nucleus , Autophagie
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