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ING1 ELISA Kit

ING1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883452
  • Target Alle ING1 ELISA Kits anzeigen
    ING1 (Inhibitor of Growth Family, Member 1 (ING1))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human ING1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes .
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle ING1 ELISA Kits anzeigen
    ING1 (Inhibitor of Growth Family, Member 1 (ING1))
    Andere Bezeichnung
    ING1 (ING1 Produkte)
    Synonyme
    p24ING1c ELISA Kit, p33 ELISA Kit, p33ING1 ELISA Kit, p33ING1b ELISA Kit, p47 ELISA Kit, p47ING1a ELISA Kit, p33ING1c ELISA Kit, zgc:136918 ELISA Kit, ING1 ELISA Kit, 2610028J21Rik ELISA Kit, AA407184 ELISA Kit, AI875420 ELISA Kit, mING1h ELISA Kit, p33Ing1 ELISA Kit, p37Ing1b ELISA Kit, xing1 ELISA Kit, inhibitor of growth family member 1 ELISA Kit, inhibitor of growth family, member 1 ELISA Kit, inhibitor of growth family member 1 S homeolog ELISA Kit, ING1 ELISA Kit, Ing1 ELISA Kit, ing1 ELISA Kit, ing1.S ELISA Kit
    Gen-ID
    3621
    UniProt
    Q9UK53
    Pathways
    Protein targeting to Nucleus, Autophagie
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