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LAMP1 ELISA Kit

LAMP1 Reaktivität: Human Colorimetric Sandwich ELISA 1.229-300 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883572
  • Target Alle LAMP1 ELISA Kits anzeigen
    LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))
    Reaktivität
    • 5
    • 3
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.229-300 ng/mL
    Untere Nachweisgrenze
    1.229 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human LAMP1/CD107a ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human LAMP1.
    Sensitivität
    1.0 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle LAMP1 ELISA Kits anzeigen
    LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))
    Andere Bezeichnung
    LAMP1 (LAMP1 Produkte)
    Synonyme
    CD107a ELISA Kit, LAMPA ELISA Kit, LGP120 ELISA Kit, AI196048 ELISA Kit, Lamp-1 ELISA Kit, LEP100 ELISA Kit, LAMP-1 ELISA Kit, LGP-A ELISA Kit, Lgpa ELISA Kit, Lysosome-associated membrane protein 1 ELISA Kit, cd107a ELISA Kit, lamp-1 ELISA Kit, lamp1 ELISA Kit, lampa ELISA Kit, lgp120 ELISA Kit, rexp76 ELISA Kit, lysosomal associated membrane protein 1 ELISA Kit, lysosomal-associated membrane protein 1 ELISA Kit, lysosomal-associated membrane protein 1 L homeolog ELISA Kit, LAMP1 ELISA Kit, Lamp1 ELISA Kit, lamp1.L ELISA Kit
    Gen-ID
    3916
    UniProt
    P11279
    Pathways
    Autophagie
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