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LGMN ELISA Kit

LGMN Reaktivität: Human Colorimetric Sandwich ELISA 28.7-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883595
  • Target Alle LGMN ELISA Kits anzeigen
    LGMN (Legumain (LGMN))
    Reaktivität
    • 5
    • 5
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    28.7-7.000 pg/mL
    Untere Nachweisgrenze
    28.7 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human Legumain ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 2B4 (CD244), ADAM-9, Angiopoietin-2, APRIL, BMP-2, BMP-9, C5a, Cathepsin L, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha / beta R2, IGF-2, IGF-2 R, IL-1 R6 (IL-1 Rrp2), IL-24, IL-33 (IL-1 F11), Kallikrein 14.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples10 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle LGMN ELISA Kits anzeigen
    LGMN (Legumain (LGMN))
    Andere Bezeichnung
    Legumain (LGMN Produkte)
    Synonyme
    AEP ELISA Kit, LGMN1 ELISA Kit, PRSC1 ELISA Kit, AI746452 ELISA Kit, AU022324 ELISA Kit, Prsc1 ELISA Kit, MGC64351 ELISA Kit, cb173 ELISA Kit, cb460 ELISA Kit, sb:cb173 ELISA Kit, zgc:76953 ELISA Kit, wu:fa98g05 ELISA Kit, wu:fj92g08 ELISA Kit, lgmn ELISA Kit, MGC88980 ELISA Kit, DELTA VACUOLAR PROCESSING ENZYME ELISA Kit, DELTAVPE ELISA Kit, delta vacuolar processing enzyme ELISA Kit, legumain ELISA Kit, legumain L homeolog ELISA Kit, delta vacuolar processing enzyme ELISA Kit, Legumain ELISA Kit, LGMN ELISA Kit, Lgmn ELISA Kit, lgmn.L ELISA Kit, lgmn ELISA Kit, DELTA-VPE ELISA Kit, MBOO_RS07785 ELISA Kit, Odosp_3582 ELISA Kit, LOC569153 ELISA Kit
    Gen-ID
    5641
    UniProt
    Q99538
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
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