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LRIG3 ELISA Kit
LRIG3
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883640
Produktdetails LRIG3 ELISA Kit
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Target
Alle LRIG3 Produkte
LRIG3
(Leucine-Rich Repeats and Immunoglobulin-Like Domains 3 (LRIG3))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human LRIG3 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Human LRIG3
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu LRIG3
(ausblenden)
Target
Alle LRIG3 Produkte
LRIG3
(Leucine-Rich Repeats and Immunoglobulin-Like Domains 3 (LRIG3))
Andere Bezeichnung
LRIG3 (LRIG3 Produkte )
Synonyme
LRIG3 ELISA Kit, lrig3 ELISA Kit, GB17149 ELISA Kit, LIG3 ELISA Kit, 9030421L11Rik ELISA Kit, 9130004I02Rik ELISA Kit, 9430095K15Rik ELISA Kit, mKIAA3016 ELISA Kit, RGD1561255 ELISA Kit, leucine rich repeats and immunoglobulin like domains 3 ELISA Kit, leucine-rich repeats and immunoglobulin-like domains 3 ELISA Kit, leucine-rich repeats and immunoglobulin-like domains protein 3 ELISA Kit, leucine-rich repeats and immunoglobulin like domains 3 S homeolog ELISA Kit, leucine-rich repeats and immunoglobulin like domains 3 ELISA Kit, LRIG3 ELISA Kit, lrig3 ELISA Kit, LOC726128 ELISA Kit, lrig3.S ELISA Kit, Lrig3 ELISA Kit
Gen-ID
121227
UniProt
Q6UXM1
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