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ST14 ELISA Kit
ST14
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883709
Produktdetails ST14 ELISA Kit
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Target
Alle ST14 ELISA Kits anzeigen
ST14
(Suppression of Tumorigenicity 14 (Colon Carcinoma) (ST14))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Human Matriptase/ST14 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human Matriptase.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu ST14
(ausblenden)
Target
Alle ST14 ELISA Kits anzeigen
ST14
(Suppression of Tumorigenicity 14 (Colon Carcinoma) (ST14))
Andere Bezeichnung
Matriptase (ST14 Produkte )
Synonyme
HAI ELISA Kit, MT-SP1 ELISA Kit, MTSP1 ELISA Kit, PRSS14 ELISA Kit, SNC19 ELISA Kit, TADG15 ELISA Kit, TMPRSS14 ELISA Kit, XMT-SP1 ELISA Kit, hai ELISA Kit, mt-sp1 ELISA Kit, mtsp1 ELISA Kit, prss14 ELISA Kit, snc19 ELISA Kit, st14 ELISA Kit, st14a ELISA Kit, tadg15 ELISA Kit, tmprss1 ELISA Kit, Epithin ELISA Kit, Prss14 ELISA Kit, Tmprss14 ELISA Kit, mCAP3 ELISA Kit, matriptase ELISA Kit, suppression of tumorigenicity 14 ELISA Kit, suppression of tumorigenicity 14 L homeolog ELISA Kit, suppression of tumorigenicity 14 (colon carcinoma) ELISA Kit, ST14 ELISA Kit, st14.L ELISA Kit, St14 ELISA Kit
Gen-ID
6768
UniProt
Q9Y5Y6
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