Myoglobin ELISA Kit
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- Target Alle Myoglobin (MB) ELISA Kits anzeigen
- Myoglobin (MB)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.82-200 ng/mL
- Untere Nachweisgrenze
- 0.82 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Human Myoglobin ELISA Kit for serum, plasma, and cell culture supernatant samples.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair detects human Myoglobin. Other species not determined.
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product MB ELISA Kit
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples2 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Myoglobin (MB) ELISA Kits anzeigen
- Myoglobin (MB)
- Andere Bezeichnung
- Myoglobin (MB Produkte)
- Synonyme
- PVALB ELISA Kit, AI325109 ELISA Kit, zgc:65819 ELISA Kit, zgc:77764 ELISA Kit, MB ELISA Kit, DKFZp468H096 ELISA Kit, myg ELISA Kit, mb ELISA Kit, MYF4 ELISA Kit, bHLHc3 ELISA Kit, myo ELISA Kit, Myoglobin ELISA Kit, myoglobin ELISA Kit, myogenin ELISA Kit, MB ELISA Kit, Mb ELISA Kit, mb ELISA Kit, myg ELISA Kit, Myog ELISA Kit
- Gen-ID
- 4151
- UniProt
- P02144
- Pathways
- Brown Fat Cell Differentiation
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