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nectin-3 ELISA Kit
NECTIN3
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883874
Produktdetails nectin-3 ELISA Kit
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Target
Alle nectin-3 (NECTIN3) ELISA Kits anzeigen
nectin-3 (NECTIN3)
(Nectin cell adhesion molecule 3 (NECTIN3))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Nectin-3 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human Nectin-3. Other species not yet determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu nectin-3
(ausblenden)
Target
Alle nectin-3 (NECTIN3) ELISA Kits anzeigen
nectin-3 (NECTIN3)
(Nectin cell adhesion molecule 3 (NECTIN3))
Andere Bezeichnung
Nectin-3 (NECTIN3 Produkte )
Synonyme
PVRL3 ELISA Kit, CD113 ELISA Kit, CDW113 ELISA Kit, NECTIN-3 ELISA Kit, PPR3 ELISA Kit, PRR3 ELISA Kit, PVRR3 ELISA Kit, 2610301B19Rik ELISA Kit, 3000002N23Rik ELISA Kit, 4921513D19Rik ELISA Kit, AA407785 ELISA Kit, AU016832 ELISA Kit, AW538082 ELISA Kit, nectin cell adhesion molecule 3 ELISA Kit, poliovirus receptor-related 3 ELISA Kit, Nectin3 ELISA Kit, NECTIN3 ELISA Kit, PVRL3 ELISA Kit, nectin3 ELISA Kit
Gen-ID
25945
UniProt
Q9NQS3
Pathways
Cell-Cell Junction Organization
Zuletzt angesehen
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