NPTXR ELISA Kit

Produktnummer ABIN4883974

Produktdetails NPTXR ELISA Kit

Target: NPTXR (Neuronal Pentraxin Receptor (NPTXR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.410-100 ng/mL

Untere Nachweisgrenze: 0.410 ng/mL

Applikation: ELISA

Verwendungszweck: Human NPTXR ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human NPTXR.

Sensitivität: 0.4 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu NPTXR

Target: NPTXR (Neuronal Pentraxin Receptor (NPTXR))

Andere Bezeichnung: NPTXR (NPTXR Produkte)

Synonyme: NPR ELISA Kit, 1200009K17Rik ELISA Kit, 1700036C17Rik ELISA Kit, 5730406O18Rik ELISA Kit, AI452369 ELISA Kit, AI785356 ELISA Kit, D15Bwg0580e ELISA Kit, Npcd ELISA Kit, Npr ELISA Kit, npr ELISA Kit, nptxr ELISA Kit, si:dkeyp-20e4.1 ELISA Kit, zgc:158178 ELISA Kit, neuronal pentraxin receptor ELISA Kit, neuronal pentraxin receptor L homeolog ELISA Kit, neuronal pentraxin receptor b ELISA Kit, NPTXR ELISA Kit, Nptxr ELISA Kit, nptxr.L ELISA Kit, nptxrb ELISA Kit

Gen-ID: 23467

UniProt: O95502