RPS6KB1 ELISA Kit

Produktnummer ABIN4884071

Produktdetails RPS6KB1 ELISA Kit

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human p70 S6 Kinase ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Andere Bezeichnung: p70 S6 Kinase (RPS6KB1 Produkte)

Synonyme: PS6K ELISA Kit, S6K ELISA Kit, S6K-beta-1 ELISA Kit, S6K1 ELISA Kit, STK14A ELISA Kit, p70 S6KA ELISA Kit, p70(S6K)-alpha ELISA Kit, p70-S6K ELISA Kit, p70-alpha ELISA Kit, 10539 ELISA Kit, 7084 ELISA Kit, CG10539 ELISA Kit, DS6K ELISA Kit, Dmel\\CG10539 ELISA Kit, Dp70S6k ELISA Kit, Dp70[s6k] ELISA Kit, Dp70s6k ELISA Kit, S6k ELISA Kit, dS6K ELISA Kit, dS6k ELISA Kit, dp70[S6k] ELISA Kit, dp70s6k ELISA Kit, dps6k ELISA Kit, ds6k ELISA Kit, fs(3)07084 ELISA Kit, l(3)07084 ELISA Kit, p70 S6K ELISA Kit, p70/S6K ELISA Kit, p70S6K ELISA Kit, p70[S6 kinase] ELISA Kit, p70[S6K] ELISA Kit, p70[S6k] ELISA Kit, p70[S6kinase] ELISA Kit, p70s6K ELISA Kit, s6k ELISA Kit, s6k11 ELISA Kit, 2610318I15Rik ELISA Kit, 4732464A07Rik ELISA Kit, 70kDa ELISA Kit, AA959758 ELISA Kit, AI256796 ELISA Kit, AI314060 ELISA Kit, p70/85s6k ELISA Kit, p70s6k ELISA Kit, p70s6k-A ELISA Kit, p70-s6k ELISA Kit, ps6k ELISA Kit, rps6kb1 ELISA Kit, rps6kb1-A ELISA Kit, s6K1 ELISA Kit, stk14a ELISA Kit, fc51h01 ELISA Kit, wu:fc51h01 ELISA Kit, zgc:55713 ELISA Kit, ribosomal protein S6 kinase B1 ELISA Kit, Ribosomal protein S6 kinase ELISA Kit, ribosomal protein S6 kinase, polypeptide 1 ELISA Kit, ribosomal protein S6 kinase B1 L homeolog ELISA Kit, ribosomal protein S6 kinase B1 S homeolog ELISA Kit, ribosomal protein S6 kinase b, polypeptide 1b ELISA Kit, RPS6KB1 ELISA Kit, S6k ELISA Kit, Rps6kb1 ELISA Kit, rps6kb1.L ELISA Kit, rps6kb1.S ELISA Kit, rps6kb1b ELISA Kit

Gen-ID: 6198

UniProt: P23443

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effekt