PAM ELISA Kit

Produktnummer ABIN4884076

Produktdetails PAM ELISA Kit

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human PAM ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Andere Bezeichnung: PAM (PAM Produkte)

Synonyme: PAL ELISA Kit, PHM ELISA Kit, pal ELISA Kit, phm ELISA Kit, pamb ELISA Kit, CG3832 ELISA Kit, Dmel\\CG3832 ELISA Kit, dPHM ELISA Kit, AE-I ELISA Kit, PAL-A ELISA Kit, PHM-A ELISA Kit, pama ELISA Kit, si:dkeyp-35f12.2 ELISA Kit, peptidylglycine alpha-amidating monooxygenase ELISA Kit, peptidylglycine alpha-amidating monooxygenase S homeolog ELISA Kit, Peptidylglycine-alpha-hydroxylating monooxygenase ELISA Kit, peptidylglycine alpha-amidating monooxygenase L homeolog ELISA Kit, PAM ELISA Kit, Pam ELISA Kit, pam.S ELISA Kit, CHLREDRAFT_150435 ELISA Kit, sce7070 ELISA Kit, pam ELISA Kit, Phm ELISA Kit, pam.L ELISA Kit

Gen-ID: 5066

UniProt: P19021