CoREST ELISA Kit

Produktnummer ABIN4884324

Produktdetails CoREST ELISA Kit

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human RCOR1/CoREST ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CoREST

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Andere Bezeichnung: RCOR1 (RCOR1 Produkte)

Synonyme: COREST ELISA Kit, RCOR ELISA Kit, 5730409O11 ELISA Kit, 6720480E22Rik ELISA Kit, AU042633 ELISA Kit, D12Wsu95e ELISA Kit, mKIAA0071 ELISA Kit, rcor1 ELISA Kit, xcoREST ELISA Kit, RCOR1 ELISA Kit, im:6904264 ELISA Kit, si:dkey-46n18.7 ELISA Kit, zgc:158607 ELISA Kit, RGD1305743 ELISA Kit, REST corepressor 1 ELISA Kit, REST corepressor 1 L homeolog ELISA Kit, RCOR1 ELISA Kit, Rcor1 ELISA Kit, rcor1.L ELISA Kit, rcor1 ELISA Kit

Gen-ID: 23186

UniProt: Q9UKL0

Pathways: Regulation of Hormone Metabolic Process, Chromatin Binding