S100A1 ELISA Kit

Produktnummer ABIN4884389

Produktdetails S100A1 ELISA Kit

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.82-200 ng/mL

Untere Nachweisgrenze: 0.82 ng/mL

Applikation: ELISA

Verwendungszweck: Human S100 A1 ELISA Kit for serum, plasma, and cell culture supernatant samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human S100A1. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Andere Bezeichnung: S100 A1 (S100A1 Produkte)

Synonyme: S100 ELISA Kit, S100-alpha ELISA Kit, S100A ELISA Kit, S100A1 ELISA Kit, DKFZp468K0511 ELISA Kit, AI266795 ELISA Kit, S100a ELISA Kit, S100 calcium binding protein A1 ELISA Kit, S100A1 ELISA Kit, S100a1 ELISA Kit, s100a1 ELISA Kit

Gen-ID: 6271

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteine