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SIRT2 ELISA Kit
SIRT2
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884558
Produktdetails SIRT2 ELISA Kit
(ausblenden)
Target
Alle SIRT2 ELISA Kits anzeigen
SIRT2
(Sirtuin 2 (SIRT2))
Reaktivität
Alle Reaktivitäten für SIRT2 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Sirtuin 2 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Sirtuin 2 (SIRT2) ELISA Kit
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Reaktivität: Maus
Colorimetric
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0.15 ng/mL - 10 ng/mL
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sirtuin 2 (SIRT2) ELISA Kit
SIRT2
Reaktivität: Maus
Colorimetric
Sandwich ELISA
1.56 ng/mL - 100 ng/mL
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sirtuin 2 (SIRT2) ELISA Kit
SIRT2
Reaktivität: Human
Colorimetric
Sandwich ELISA
1.56 ng/mL - 100 ng/mL
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sirtuin 2 (SIRT2) ELISA Kit
SIRT2
Reaktivität: Human
Colorimetric
Sandwich ELISA
1.56 ng/mL - 100 ng/mL
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anwendungsinformationen
(ausblenden)
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SIRT2
(ausblenden)
Target
Alle SIRT2 ELISA Kits anzeigen
SIRT2
(Sirtuin 2 (SIRT2))
Andere Bezeichnung
Sirtuin 2 (SIRT2 Produkte )
Gen-ID
22933
UniProt
Q8IXJ6
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