SMAD4 ELISA Kit

Produktnummer ABIN4884583

Produktdetails SMAD4 ELISA Kit

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human SMAD4 ELISA Kit for Cell Culture Supernatants.

Proben: Cell Culture Supernatant

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human SMAD4.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: Smad4 (SMAD4 Produkte)

Synonyme: smad4 ELISA Kit, DPC4 ELISA Kit, JIP ELISA Kit, MADH4 ELISA Kit, MYHRS ELISA Kit, AW743858 ELISA Kit, D18Wsu70e ELISA Kit, Madh4 ELISA Kit, XSmad4alpha ELISA Kit, Xsmad4 ELISA Kit, dpc4 ELISA Kit, jip ELISA Kit, madh4 ELISA Kit, xsmad4a ELISA Kit, SMAD family member 4 ELISA Kit, Smad4 protein ELISA Kit, SMAD family member 4, gene 1 L homeolog ELISA Kit, SMAD4 ELISA Kit, smad4 ELISA Kit, Smad4 ELISA Kit, smad4.1.L ELISA Kit

Gen-ID: 4089

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie