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Src ELISA Kit
Src
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884647
Produktdetails Src ELISA Kit
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Target
Alle Src ELISA Kits anzeigen
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
Reaktivität
Alle Reaktivitäten für Src ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Src ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu Src
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Target
Alle Src ELISA Kits anzeigen
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
Andere Bezeichnung
Src (Src Produkte )
Synonyme
AW259666 ELISA Kit, pp60c-src ELISA Kit, C-src1 ELISA Kit, CG7524 ELISA Kit, D-Src64B ELISA Kit, D-src ELISA Kit, DSRC64 ELISA Kit, DSrc ELISA Kit, DSrc64 ELISA Kit, DSrc64B ELISA Kit, Dm SRC1 ELISA Kit, DmHD-358 ELISA Kit, Dmel\CG7524 ELISA Kit, Dsrc ELISA Kit, Dsrc64 ELISA Kit, Dsrc64B ELISA Kit, HD-358 ELISA Kit, MRE5 ELISA Kit, SRC 64B ELISA Kit, Src ELISA Kit, Src1 ELISA Kit, Src64 ELISA Kit, c-src ELISA Kit, dSrc ELISA Kit, dsrc ELISA Kit, src ELISA Kit, src1 ELISA Kit, src64 ELISA Kit, src64B ELISA Kit, src64b ELISA Kit, ASV ELISA Kit, SRC1 ELISA Kit, c-SRC ELISA Kit, p60-Src ELISA Kit, c-Src ELISA Kit, PP60C-SCR ELISA Kit, SDR ELISA Kit, SRC proto-oncogene, non-receptor tyrosine kinase ELISA Kit, uncharacterized LOC5574802 ELISA Kit, proto-oncogene tyrosine-protein kinase src ELISA Kit, Rous sarcoma oncogene ELISA Kit, Src oncogene at 64B ELISA Kit, SRC ELISA Kit, LOC5574802 ELISA Kit, CpipJ_CPIJ010659 ELISA Kit, Src ELISA Kit, Src64B ELISA Kit
Substanzklasse
Viral Protein
Gen-ID
6714
UniProt
P12931
Pathways
JAK-STAT Signalweg , Neurotrophin Signalübertragung , Intracellular Steroid Hormone Receptor Signaling Pathway , Regulation of Intracellular Steroid Hormone Receptor Signaling , Cellular Response to Molecule of Bacterial Origin , Cell-Cell Junction Organization , Regulation of Carbohydrate Metabolic Process , Autophagie , CXCR4-mediated Signaling Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Smooth Muscle Cell Migration , Negative Regulation of intrinsic apoptotic Signaling , Platelet-derived growth Factor Receptor Signaling , Thromboxane A2 Receptor Signaling , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling
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