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Syntaxin 6 ELISA Kit
STX6
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884683
Produktdetails Syntaxin 6 ELISA Kit
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Target
Alle Syntaxin 6 (STX6) ELISA Kits anzeigen
Syntaxin 6 (STX6)
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Syntaxin 6 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu Syntaxin 6
(ausblenden)
Target
Alle Syntaxin 6 (STX6) ELISA Kits anzeigen
Syntaxin 6 (STX6)
Andere Bezeichnung
Syntaxin 6 (STX6 Produkte )
Synonyme
CG7736 ELISA Kit, CT23545 ELISA Kit, Dm Syx6 ELISA Kit, DmSyx6 ELISA Kit, Dmel\\CG7736 ELISA Kit, SYN6 ELISA Kit, SYX6/10 ELISA Kit, Syx ELISA Kit, MGC78971 ELISA Kit, MGC132090 ELISA Kit, STX6 ELISA Kit, fb51b08 ELISA Kit, wu:fb51b08 ELISA Kit, zgc:110658 ELISA Kit, si:dkey-121a11.2 ELISA Kit, LOC734102 ELISA Kit, Syx6 ELISA Kit, Bmsyx6 ELISA Kit, Syntaxin-6 ELISA Kit, 2310039E05Rik ELISA Kit, 2410005I16Rik ELISA Kit, AA437559 ELISA Kit, AI415714 ELISA Kit, AI845861 ELISA Kit, Syntaxin 6 ELISA Kit, syntaxin 6 L homeolog ELISA Kit, syntaxin 6 ELISA Kit, Syx6 ELISA Kit, stx6.L ELISA Kit, STX6 ELISA Kit, stx6 ELISA Kit, Stx6 ELISA Kit
Gen-ID
10228
UniProt
O43752
Pathways
Synaptic Vesicle Exocytosis
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