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TNFRSF12A ELISA Kit
TNFRSF12A
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884960
Produktdetails TNFRSF12A ELISA Kit
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Target
Alle TNFRSF12A ELISA Kits anzeigen
TNFRSF12A
(Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
Reaktivität
Alle Reaktivitäten für TNFRSF12A ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human TWEAK R (TNFRSF12) ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human TWEAK R. Other species not yet determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TNFRSF12A
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Target
Alle TNFRSF12A ELISA Kits anzeigen
TNFRSF12A
(Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
Andere Bezeichnung
TWEAK R (TNFRSF12A Produkte )
Synonyme
CD266 ELISA Kit, FN14 ELISA Kit, TWEAKR ELISA Kit, AI255180 ELISA Kit, C87282 ELISA Kit, Fn14 ELISA Kit, HPIP ELISA Kit, TWEAK-R ELISA Kit, TweakR ELISA Kit, TNF receptor superfamily member 12A ELISA Kit, tumor necrosis factor receptor superfamily, member 12a ELISA Kit, TNFRSF12A ELISA Kit, Tnfrsf12a ELISA Kit
UniProt
Q9NP84
Pathways
Apoptose , Regulation of Cell Size
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