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VAPB ELISA Kit
VAPB
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885020
Produktdetails VAPB ELISA Kit
(ausblenden)
Target
Alle VAPB ELISA Kits anzeigen
VAPB
(VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))
Reaktivität
Alle Reaktivitäten für VAPB ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human VAP-B ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu VAPB
(ausblenden)
Target
Alle VAPB ELISA Kits anzeigen
VAPB
(VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))
Andere Bezeichnung
VAP-B (VAPB Produkte )
Synonyme
ALS8 ELISA Kit, VAMP-B ELISA Kit, VAP-B ELISA Kit, AI225786 ELISA Kit, AI840687 ELISA Kit, AI848259 ELISA Kit, D2Abb2e ELISA Kit, R75548 ELISA Kit, VAP33b ELISA Kit, Vamp33b ELISA Kit, fc05d09 ELISA Kit, wu:fa96d05 ELISA Kit, wu:fc05d09 ELISA Kit, zgc:66045 ELISA Kit, vamp-b ELISA Kit, vamp-c ELISA Kit, vap-b ELISA Kit, vap-c ELISA Kit, vapb ELISA Kit, VAMP associated protein B and C ELISA Kit, vesicle-associated membrane protein-associated protein B/C ELISA Kit, vesicle-associated membrane protein, associated protein B and C ELISA Kit, VAMP (vesicle-associated membrane protein)-associated protein B and C ELISA Kit, VAMP (vesicle-associated membrane protein)-associated protein B and C L homeolog ELISA Kit, VAPB ELISA Kit, Tsp_07972 ELISA Kit, Vapb ELISA Kit, vapb ELISA Kit, vapb.L ELISA Kit
Gen-ID
9217
UniProt
O95292
Pathways
ER-Nucleus Signaling
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