CD59 ELISA Kit
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- Target Alle CD59 ELISA Kits anzeigen
- CD59
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Custom Human CD59 SpeedELISA Kit.
- Marke
- SpeedELISA
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Produktmerkmale
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product CD59 ELISA Kit
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- Probenmenge
- 100 μL
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - Haltbarkeit
- 6 months
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- Target Alle CD59 ELISA Kits anzeigen
- CD59
- Andere Bezeichnung
- CD59 (CD59 Produkte)
- Synonyme
- 16.3A5 ELISA Kit, 1F5 ELISA Kit, EJ16 ELISA Kit, EJ30 ELISA Kit, EL32 ELISA Kit, G344 ELISA Kit, HRF-20 ELISA Kit, HRF20 ELISA Kit, MAC-IP ELISA Kit, MACIF ELISA Kit, MEM43 ELISA Kit, MIC11 ELISA Kit, MIN1 ELISA Kit, MIN2 ELISA Kit, MIN3 ELISA Kit, MIRL ELISA Kit, MSK21 ELISA Kit, p18-20 ELISA Kit, Cd59a ELISA Kit, Cd59b ELISA Kit, MACIP ELISA Kit, CD59 molecule (CD59 blood group) ELISA Kit, CD59 molecule ELISA Kit, CD59 ELISA Kit, Cd59 ELISA Kit
- Gen-ID
- 966
- UniProt
- P13987
- Pathways
- Komplementsystem
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