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EGFR ELISA Kit
EGFR
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4885202
Produktdetails EGFR ELISA Kit
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Target
Alle EGFR ELISA Kits anzeigen
EGFR
(Epidermal Growth Factor Receptor (EGFR))
Reaktivität
Alle Reaktivitäten für EGFR ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human EGFR SpeedELISA Kit.
Marke
SpeedELISA
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Produktmerkmale
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Testdauer
3 h
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Haltbarkeit
6 months
Details zu EGFR
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Target
Alle EGFR ELISA Kits anzeigen
EGFR
(Epidermal Growth Factor Receptor (EGFR))
Andere Bezeichnung
EGFR (EGFR Produkte )
Synonyme
C-erb ELISA Kit, CG10079 ELISA Kit, D-EGFR ELISA Kit, D-Egf ELISA Kit, DEGFR ELISA Kit, DER ELISA Kit, DER flb ELISA Kit, DER/EGFR ELISA Kit, DER/faint little ball ELISA Kit, DER/top ELISA Kit, DER/torpedo ELISA Kit, DER1 ELISA Kit, DEgfr ELISA Kit, Degfr ELISA Kit, Der ELISA Kit, DmHD-33 ELISA Kit, Dmel\\CG10079 ELISA Kit, EFG-R ELISA Kit, EGF-R ELISA Kit, EGFR ELISA Kit, EGFr ELISA Kit, EGfr ELISA Kit, EK2-6 ELISA Kit, Egf ELISA Kit, Egf-r ELISA Kit, EgfR ELISA Kit, El ELISA Kit, Elp ELISA Kit, Elp-1 ELISA Kit, Elp-B1 ELISA Kit, Elp-B1RB1 ELISA Kit, HD-33 ELISA Kit, TOP ELISA Kit, Torpedo/DER ELISA Kit, Torpedo/Egfr ELISA Kit, c-erbB ELISA Kit, d-egf-r ELISA Kit, dEGFR ELISA Kit, dEGFR1 ELISA Kit, dEgfr ELISA Kit, der ELISA Kit, egfr ELISA Kit, flb ELISA Kit, l(2)05351 ELISA Kit, l(2)09261 ELISA Kit, l(2)57DEFa ELISA Kit, l(2)57EFa ELISA Kit, l(2)57Ea ELISA Kit, mor1 ELISA Kit, top ELISA Kit, top/DER ELISA Kit, top/flb ELISA Kit, torpedo/Egfr ELISA Kit, torpedo/egfr ELISA Kit, EGFR12 ELISA Kit, EGFR15 ELISA Kit, egfr1 ELISA Kit, Erbb2 ELISA Kit, ERBB ELISA Kit, ERBB1 ELISA Kit, HER1 ELISA Kit, PIG61 ELISA Kit, mENA ELISA Kit, ErbB-1 ELISA Kit, Errp ELISA Kit, 9030024J15Rik ELISA Kit, AI552599 ELISA Kit, Erbb ELISA Kit, Errb1 ELISA Kit, Wa5 ELISA Kit, wa-2 ELISA Kit, wa2 ELISA Kit, epidermal growth factor receptor ELISA Kit, Epidermal growth factor receptor ELISA Kit, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) ELISA Kit, EGFR ELISA Kit, Egfr ELISA Kit, egfra ELISA Kit, egfr1 ELISA Kit, LOC5564544 ELISA Kit
Gen-ID
1956
UniProt
P00533
Pathways
NF-kappaB Signalweg , RTK Signalweg , Fc-epsilon Rezeptor Signalübertragung , EGFR Signaling Pathway , Neurotrophin Signalübertragung , Stem Cell Maintenance , Hepatitis C , Positive Regulation of Response to DNA Damage Stimulus , Interaction of EGFR with phospholipase C-gamma , Thromboxane A2 Receptor Signaling , EGFR Downregulation , S100 Proteine
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