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PAI1 ELISA Kit
SERPINE1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4885297
Produktdetails PAI1 ELISA Kit
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Target
Alle PAI1 (SERPINE1) ELISA Kits anzeigen
PAI1 (SERPINE1)
(Plasminogen Activator Inhibitor 1 (SERPINE1))
Reaktivität
Alle Reaktivitäten für PAI1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human PAI-1 SpeedELISA Kit.
Marke
SpeedELISA
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Produktmerkmale
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Testdauer
3 h
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Haltbarkeit
6 months
Details zu PAI1
(ausblenden)
Target
Alle PAI1 (SERPINE1) ELISA Kits anzeigen
PAI1 (SERPINE1)
(Plasminogen Activator Inhibitor 1 (SERPINE1))
Andere Bezeichnung
PAI-1 (SERPINE1 Produkte )
Synonyme
PAI ELISA Kit, PAI-1 ELISA Kit, PAI1 ELISA Kit, PLANH1 ELISA Kit, SERPINE1 ELISA Kit, si:ch211-138a11.1 ELISA Kit, Planh1 ELISA Kit, PAI1A ELISA Kit, Pai1 ELISA Kit, Pai1aa ELISA Kit, Planh ELISA Kit, RATPAI1A ELISA Kit, serpin family E member 1 ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog ELISA Kit, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade E, member 1 ELISA Kit, SERPINE1 ELISA Kit, serpine1.L ELISA Kit, serpine1 ELISA Kit, Serpine1 ELISA Kit
Gen-ID
5054
UniProt
P05121
Pathways
p53 Signalweg , Cellular Response to Molecule of Bacterial Origin , Carbohydrate Homeostasis , Autophagie , Smooth Muscle Cell Migration
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