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CCL28 ELISA Kit

CCL28 Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885454
  • Target Alle CCL28 ELISA Kits anzeigen
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    Reaktivität
    • 5
    • 4
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Mouse Chemokine (C-C motif) Ligand 28 (MEC) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Mouse Chemokine (C-C motif) Ligand 28 (MEC)
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle CCL28 ELISA Kits anzeigen
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    Andere Bezeichnung
    CCL28 (CCL28 Produkte)
    Synonyme
    Scya28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, nrpb ELISA Kit, enc-1 ELISA Kit, pig10 ELISA Kit, Encr-3 ELISA Kit, klhl35 ELISA Kit, Xencr-3 ELISA Kit, tp53i10 ELISA Kit, LOC100303767 ELISA Kit, CCK1 ELISA Kit, MEC ELISA Kit, SCYA28 ELISA Kit, C-C motif chemokine ligand 28 ELISA Kit, ectodermal-neural cortex 1 (with BTB domain) ELISA Kit, C-C motif chemokine 28 ELISA Kit, chemokine (C-C motif) ligand 28 ELISA Kit, Ccl28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, enc1 ELISA Kit, LOC100303767 ELISA Kit
    Gen-ID
    56838
    UniProt
    Q9JIL2
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