Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

ROBO1 ELISA Kit

ROBO1 Reaktivität: Ratte Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885893
  • Target Alle ROBO1 ELISA Kits anzeigen
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Reaktivität
    • 4
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Rat ROBO1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes rat ROBO1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften ROBO1 ELISA Kit
    Top Product
    Discover our top product ROBO1 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle ROBO1 ELISA Kits anzeigen
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Andere Bezeichnung
    ROBO1 (ROBO1 Produkte)
    Synonyme
    DUTT1 ELISA Kit, SAX3 ELISA Kit, AW494633 ELISA Kit, AW742721 ELISA Kit, Gm310 ELISA Kit, ROBO1 ELISA Kit, dutt1 ELISA Kit, roundabout ELISA Kit, sax3 ELISA Kit, MGC122264 ELISA Kit, roundabout-1 ELISA Kit, roundabout guidance receptor 1 ELISA Kit, roundabout, axon guidance receptor, homolog 1 (Drosophila) ELISA Kit, roundabout guidance receptor 1 L homeolog ELISA Kit, ROBO1 ELISA Kit, Robo1 ELISA Kit, robo1 ELISA Kit, robo1.L ELISA Kit
    Gen-ID
    58946
    UniProt
    O55005
    Pathways
    Positive Regulation of Endopeptidase Activity
Sie sind hier:
Kundenservice