×
Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.
DIABLO ELISA Kit
DIABLO
Reaktivität: Ratte
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885896
Produktdetails DIABLO ELISA Kit
(ausblenden)
Target
Alle DIABLO ELISA Kits anzeigen
DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
Reaktivität
Alle Reaktivitäten für DIABLO ELISA Kits
Ratte
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Rat SMAC ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Rat SMAC.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Featured
Zu unserem meistverkauften DIABLO ELISA Kit
Top Product
Discover our top product DIABLO ELISA Kit
Alternativen
(anzeigen)
Anwendungsinformationen
(ausblenden)
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
(ausblenden)
Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu DIABLO
(ausblenden)
Target
Alle DIABLO ELISA Kits anzeigen
DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
Andere Bezeichnung
SMAC (DIABLO Produkte )
Synonyme
smac ELISA Kit, smac3 ELISA Kit, diablo-s ELISA Kit, 0610041G12Rik ELISA Kit, 1700006L01Rik ELISA Kit, AU040403 ELISA Kit, Smac ELISA Kit, DFNA64 ELISA Kit, SMAC ELISA Kit, DBO ELISA Kit, XSmac ELISA Kit, diablo, IAP-binding mitochondrial protein ELISA Kit, diablo IAP-binding mitochondrial protein ELISA Kit, diablo, IAP-binding mitochondrial protein L homeolog ELISA Kit, diablo ELISA Kit, Diablo ELISA Kit, DIABLO ELISA Kit, diablo.L ELISA Kit
Gen-ID
288753
UniProt
Q5RK17
Pathways
Apoptose , Caspase Kaskade in der Apoptose , Positive Regulation of Endopeptidase Activity
Zuletzt angesehen
(ausblenden)