ATM ELISA Kit

Produktnummer ABIN4885984

Produktdetails ATM ELISA Kit

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Bindungsspezifität: pSer1981, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-ATM (S1981) and Total ATM ELISA Kit. This assay semi-quantitatively measures phophorylated ATM (Ser1981) and Total ATM in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes human ATM phosphorylated at site Serine-1981 as well as total ATM.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Andere Bezeichnung: ATM (ATM Produkte)

Synonyme: ATM ELISA Kit, Atm ELISA Kit, CG6535 ELISA Kit, Dmel\\CG6535 ELISA Kit, Tefu ELISA Kit, atm ELISA Kit, atm/tefu ELISA Kit, dATM ELISA Kit, tef ELISA Kit, Xatm ELISA Kit, at1 ELISA Kit, atdc ELISA Kit, tel1 ELISA Kit, telo1 ELISA Kit, AT1 ELISA Kit, ATA ELISA Kit, ATC ELISA Kit, ATD ELISA Kit, ATDC ELISA Kit, ATE ELISA Kit, TEL1 ELISA Kit, TELO1 ELISA Kit, AI256621 ELISA Kit, C030026E19Rik ELISA Kit, telomere fusion ELISA Kit, ATM serine/threonine kinase L homeolog ELISA Kit, ATM serine/threonine kinase ELISA Kit, ataxia telangiectasia mutated ELISA Kit, ataxia telangiectasia mutated (atm) ELISA Kit, serine/threonine-protein kinase ATM ELISA Kit, tefu ELISA Kit, atm.L ELISA Kit, atm ELISA Kit, ATM ELISA Kit, EDI_100660 ELISA Kit, CpipJ_CPIJ001772 ELISA Kit, BDBG_08252 ELISA Kit, PAAG_02532 ELISA Kit, MCYG_05088 ELISA Kit, VDBG_06833 ELISA Kit, ACLA_015700 ELISA Kit, LOC5565620 ELISA Kit, MGYG_07634 ELISA Kit, PGTG_14279 ELISA Kit, Atm ELISA Kit

Gen-ID: 472

UniProt: Q13315

Pathways: p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus