CHEK2 ELISA Kit

Produktnummer ABIN4886029

Produktdetails CHEK2 ELISA Kit

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Bindungsspezifität: pThr68, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-CHK2 (T68) and Total CHK2 ELISA Kit. This assay semi-quantitatively measures phophorylated CHK2 (Thr68) and Total CHK2 in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human CHK2 phosphorylated at site Threonine-68 and Total CHK2.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: CHK2 (CHEK2 Produkte)

Synonyme: CDS1 ELISA Kit, CHK2 ELISA Kit, HuCds1 ELISA Kit, LFS2 ELISA Kit, PP1425 ELISA Kit, RAD53 ELISA Kit, hCds1 ELISA Kit, fa66f08 ELISA Kit, wu:fa66f08 ELISA Kit, zgc:55865 ELISA Kit, Cds1 ELISA Kit, HUCDS1 ELISA Kit, Rad53 ELISA Kit, Chk2 ELISA Kit, cds1 ELISA Kit, chek2 ELISA Kit, chk2 ELISA Kit, hucds1 ELISA Kit, lfs2 ELISA Kit, pp1425 ELISA Kit, rad53 ELISA Kit, checkpoint kinase 2 ELISA Kit, serine/threonine-protein kinase chk2 ELISA Kit, checkpoint kinase 2 L homeolog ELISA Kit, CHEK2 ELISA Kit, chek2 ELISA Kit, Chek2 ELISA Kit, MCYG_07308 ELISA Kit, chek2.L ELISA Kit

Gen-ID: 11200

UniProt: O96017

Pathways: p53 Signalweg, Apoptose, Zellzyklus