Insulin Receptor ELISA Kit

Produktnummer ABIN4886144

Produktdetails Insulin Receptor ELISA Kit

Target: Insulin Receptor (INSR)

Bindungsspezifität: pTyr1189, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Phospho-INSR (Y1189) and Total INSR ELISA Kit. This assay semi-quantitatively measures phophorylated INSR (Tyr1189) and Total INSR in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human INSR phosphorylated at site Tyrosine-1189 and total INSR.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu Insulin Receptor

Target: Insulin Receptor (INSR)

Andere Bezeichnung: Insulin R (INSR Produkte)

Synonyme: CD220 ELISA Kit, HHF5 ELISA Kit, 4932439J01Rik ELISA Kit, D630014A15Rik ELISA Kit, IR ELISA Kit, IR-A ELISA Kit, IR-B ELISA Kit, 18402 ELISA Kit, CG18402 ELISA Kit, DIHR ELISA Kit, DILR ELISA Kit, DIR ELISA Kit, DIRH ELISA Kit, DIRbeta ELISA Kit, DInR ELISA Kit, DInr ELISA Kit, Dir-a ELISA Kit, Dir-b ELISA Kit, Dmel\\CG18402 ELISA Kit, INR ELISA Kit, INS ELISA Kit, Inr ELISA Kit, Inr-alpha ELISA Kit, Inr-beta ELISA Kit, InsR ELISA Kit, dINR ELISA Kit, dIR ELISA Kit, dIRH ELISA Kit, dInR ELISA Kit, dInr ELISA Kit, dInsR ELISA Kit, dinr ELISA Kit, dir ELISA Kit, er10 ELISA Kit, inr ELISA Kit, insulin/insulin-like growth factor receptor ELISA Kit, l(3)05545 ELISA Kit, l(3)93Dj ELISA Kit, l(3)er10 ELISA Kit, lnR ELISA Kit, ir-A ELISA Kit, CTK-1 ELISA Kit, ir ELISA Kit, INSR ELISA Kit, NV14476 ELISA Kit, cd220 ELISA Kit, hhf5 ELISA Kit, insulin receptor ELISA Kit, Insulin-like receptor ELISA Kit, insulin receptor L homeolog ELISA Kit, INSR ELISA Kit, Insr ELISA Kit, InR ELISA Kit, LOC100122567 ELISA Kit, LOC100451802 ELISA Kit, insr.L ELISA Kit

Gen-ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signalweg, RTK Signalweg, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity