MAPK14 ELISA Kit

Produktnummer ABIN4886228

Produktdetails MAPK14 ELISA Kit

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Bindungsspezifität: pTyr

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Phosphotyrosine P38 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine P38 in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-P38.

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Andere Bezeichnung: p38 (MAPK14 Produkte)

Synonyme: CSBP ELISA Kit, CSBP1 ELISA Kit, CSBP2 ELISA Kit, CSPB1 ELISA Kit, EXIP ELISA Kit, Mxi2 ELISA Kit, PRKM14 ELISA Kit, PRKM15 ELISA Kit, RK ELISA Kit, SAPK2A ELISA Kit, p38 ELISA Kit, p38ALPHA ELISA Kit, CRK1 ELISA Kit, Csbp1 ELISA Kit, Csbp2 ELISA Kit, Exip ELISA Kit, Hog ELISA Kit, Prkm14 ELISA Kit, Prkm15 ELISA Kit, Sapk2A ELISA Kit, p38Hog ELISA Kit, p38alpha ELISA Kit, p38b ELISA Kit, zp38b ELISA Kit, MAPK14 ELISA Kit, 186F5S ELISA Kit, BG:DS00797.3 ELISA Kit, CG7393 ELISA Kit, D-p38 ELISA Kit, D-p38 MAPK ELISA Kit, D-p38b ELISA Kit, Dm p38b ELISA Kit, Dmel\\CG7393 ELISA Kit, Dmp38b ELISA Kit, Dp38 ELISA Kit, Dp38b ELISA Kit, ESTS:186F5S ELISA Kit, Mpk34C ELISA Kit, anon-sts23 ELISA Kit, dp38b ELISA Kit, p38 MAPK ELISA Kit, p38 beta ELISA Kit, p38B ELISA Kit, p38Kb ELISA Kit, p38beta ELISA Kit, Crk1 ELISA Kit, p38-alpha ELISA Kit, p38MAPK ELISA Kit, p38a ELISA Kit, csbp ELISA Kit, mapk14a ELISA Kit, mxi2 ELISA Kit, sapk2 ELISA Kit, sapk2a ELISA Kit, AP22.98 ELISA Kit, AP22_98 ELISA Kit, ATMPK14 ELISA Kit, mitogen-activated protein kinase 14 ELISA Kit, SAPK2a ELISA Kit, MAP kinase 14A ELISA Kit, MAP kinase p38a ELISA Kit, MAPK 14A ELISA Kit, fk28c03 ELISA Kit, hm:zeh1243 ELISA Kit, wu:fk28c03 ELISA Kit, zp38a ELISA Kit, P38C-CRK ELISA Kit, mitogen-activated protein kinase 14 ELISA Kit, mitogen activated protein kinase 14 ELISA Kit, mitogen-activated protein kinase 14b ELISA Kit, p38b MAP kinase ELISA Kit, mitogen-activated protein kinase 14 S homeolog ELISA Kit, mitogen-activated protein kinase 14a ELISA Kit, CRK proto-oncogene, adaptor protein ELISA Kit, MAPK14 ELISA Kit, Mapk14 ELISA Kit, mapk14b ELISA Kit, p38b ELISA Kit, mapk14.S ELISA Kit, MPK14 ELISA Kit, mapk14a ELISA Kit, CRK ELISA Kit

Gen-ID: 1432

UniProt: Q16539

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteine