RYK ELISA Kit

Produktnummer ABIN4886279

Produktdetails RYK ELISA Kit

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Bindungsspezifität: pTyr

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Phosphotyrosine RYK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine RYK in lysate samples.

Proben: Cell Lysate, Tissue Lysate

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-RYK.

Produktmerkmale:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu RYK

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Andere Bezeichnung: RYK (RYK Produkte)

Synonyme: RYK ELISA Kit, jtk5 ELISA Kit, ryk1 ELISA Kit, jtk5a ELISA Kit, d3s3195 ELISA Kit, wu:fb37b12 ELISA Kit, wu:fi05b06 ELISA Kit, zgc:158381 ELISA Kit, MGC114645 ELISA Kit, D3S3195 ELISA Kit, JTK5 ELISA Kit, JTK5A ELISA Kit, RYK1 ELISA Kit, AW536699 ELISA Kit, ERK-3 ELISA Kit, Vik ELISA Kit, receptor-like tyrosine kinase ELISA Kit, receptor-like tyrosine kinase S homeolog ELISA Kit, tyrosine-protein kinase RYK ELISA Kit, RYK ELISA Kit, ryk ELISA Kit, ryk.S ELISA Kit, LOC100541106 ELISA Kit, Ryk ELISA Kit

Gen-ID: 6259

UniProt: P34925

Pathways: RTK Signalweg, WNT Signalweg, Regulation of Cell Size