SHP1 ELISA Kit

Produktnummer ABIN4886288

Produktdetails SHP1 ELISA Kit

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Bindungsspezifität: pSer591, total

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human and Mouse Phospho-SHP1 (Ser591) and Total SHP1 ELISA Kit. This assay semi-quantitatively measures phophorylated SHP1 (Ser591) and Total SHP1 in lysate samples.

Proben: Cell Culture Lysate

Analytische Methode: Semi-Quantitative

Spezifität: This ELISA kit recognizes Human and Mouse SHP1 phosphorylated at site Serine-591 as well as total SHP1.

Produktmerkmale:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Haltbarkeit: 6 months

Details zu SHP1

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Andere Bezeichnung: SHP1 (PTPN6 Produkte)

Synonyme: HCPH ELISA Kit, HPTP1C ELISA Kit, PTP-1C ELISA Kit, SH-PTP1 ELISA Kit, SHP-1 ELISA Kit, SHP-1L ELISA Kit, SHP1 ELISA Kit, DRPLA ELISA Kit, Hcph ELISA Kit, Ptp1C ELISA Kit, me ELISA Kit, motheaten ELISA Kit, Ptph6 ELISA Kit, Shp-1 ELISA Kit, fj22f05 ELISA Kit, wu:fj22f05 ELISA Kit, zgc:55501 ELISA Kit, ptpn6 ELISA Kit, protein tyrosine phosphatase, non-receptor type 6 ELISA Kit, protein tyrosine phosphatase, non-receptor type 6 L homeolog ELISA Kit, PTPN6 ELISA Kit, Ptpn6 ELISA Kit, ptpn6 ELISA Kit, ptpn6.L ELISA Kit

Gen-ID: 5777

UniProt: P29350, P29351

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling