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DDR2 ELISA Kit

DDR2 Reaktivität: Human pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886342
  • Target Alle DDR2 ELISA Kits anzeigen
    DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
    Bindungsspezifität
    pTyr
    Reaktivität
    • 7
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Phosphotyrosine TYRO10 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine TYRO10 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-TYRO10.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle DDR2 ELISA Kits anzeigen
    DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
    Andere Bezeichnung
    TYRO10 (DDR2 Produkte)
    Synonyme
    MIG20a ELISA Kit, NTRKR3 ELISA Kit, TKT ELISA Kit, TYRO10 ELISA Kit, AW495251 ELISA Kit, Ntrkr3 ELISA Kit, tyro10 ELISA Kit, DDR2 ELISA Kit, Tyro10 ELISA Kit, ddr2 ELISA Kit, si:ch211-193c2.1 ELISA Kit, discoidin domain receptor tyrosine kinase 2 ELISA Kit, discoidin domain receptor family, member 2 ELISA Kit, discoidin domain receptor family, member 2, like ELISA Kit, DDR2 ELISA Kit, Ddr2 ELISA Kit, ddr2l ELISA Kit
    Gen-ID
    4921
    UniProt
    Q16832
    Pathways
    RTK Signalweg
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