Vasopressin ELISA Kit
-
- Target Alle Vasopressin (AVP) ELISA Kits anzeigen
- Vasopressin (AVP) (Arginine Vasopressin (AVP))
-
Reaktivität
- Human, Ratte, Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Competition ELISA
- Detektionsbereich
- 0.1-1.000 ng/mL
- Untere Nachweisgrenze
- 0.1 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Human/Mouse/Rat Neurophysin-2 EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This EIA kit is designed to detect human, mouse, and rat detect Neurophysin 2 and the propeptide Vasopressin-Neurophysin 2-copeptin.
- Sensitivität
- 1.4 ng/mL
- Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
- Featured
- Zu unserem meistverkauften AVP ELISA Kit
- Top Product
- Discover our top product AVP ELISA Kit
-
-
- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- Aufbereitung der Reagenzien
-
Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- Haltbarkeit
- 6 months
-
- Target Alle Vasopressin (AVP) ELISA Kits anzeigen
- Vasopressin (AVP) (Arginine Vasopressin (AVP))
- Andere Bezeichnung
- Neurophysin-2 (AVP Produkte)
- Synonyme
- ADH ELISA Kit, ARVP ELISA Kit, AVP-NPII ELISA Kit, AVRP ELISA Kit, VP ELISA Kit, 2610201A18Rik ELISA Kit, Akr1a4 ELISA Kit, Vp ELISA Kit, Vsp ELISA Kit, DI ELISA Kit, Vas ELISA Kit, Adh ELISA Kit, Adh1a ELISA Kit, Adh1c ELISA Kit, adh ELISA Kit, arvp ELISA Kit, avrp ELISA Kit, avp-npii ELISA Kit, copeptin ELISA Kit, vasotocin ELISA Kit, arginine vasopressin ELISA Kit, aldo-keto reductase family 1, member A1 (aldehyde reductase) ELISA Kit, alcohol dehydrogenase 1 (class I) ELISA Kit, AVP ELISA Kit, Akr1a1 ELISA Kit, Avp ELISA Kit, Adh1 ELISA Kit, avp ELISA Kit
- Gen-ID
- 24221
- UniProt
- P01186
- Pathways
- cAMP Metabolic Process
-